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Figure 3 Decreased caspase activation in PT45-P1res cells is IL-1b-dependent. (a) PT45-P1 and PT45-P1res cells were either left untreated or treated with 20 mg/ml etoposide for 48 h. Additionally, PT45-P1res cells were coincubated with 250 ng/ml IL1-RA. Cellular lysates were submitted to Western blotting using antibodies for the detection of full length and cleaved caspase-3, - 7, -8 and -9. An a-tubulin antibody was used as a control for equal protein load. (b) Cells were treated as above and analysed for caspase-3/7 activity expressed as n-fold induction of caspase-3/7 activity over basal. Means7s.d. from three independent experi- ments are shown. *Indicates Po0.05. (c) Detection of <t>cIAP1,</t> cIAP2 and XIAP in untreated cellular lysates of PT45-P1 and PT45-P1res cells by Western blotting. An a-tubulin antibody was used as a control for equal protein load.
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R&D Systems anti goat human ciap 1
Figure 3 Decreased caspase activation in PT45-P1res cells is IL-1b-dependent. (a) PT45-P1 and PT45-P1res cells were either left untreated or treated with 20 mg/ml etoposide for 48 h. Additionally, PT45-P1res cells were coincubated with 250 ng/ml IL1-RA. Cellular lysates were submitted to Western blotting using antibodies for the detection of full length and cleaved caspase-3, - 7, -8 and -9. An a-tubulin antibody was used as a control for equal protein load. (b) Cells were treated as above and analysed for caspase-3/7 activity expressed as n-fold induction of caspase-3/7 activity over basal. Means7s.d. from three independent experi- ments are shown. *Indicates Po0.05. (c) Detection of <t>cIAP1,</t> cIAP2 and XIAP in untreated cellular lysates of PT45-P1 and PT45-P1res cells by Western blotting. An a-tubulin antibody was used as a control for equal protein load.
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R&D Systems goat anti human ciap1
Figure 3 Decreased caspase activation in PT45-P1res cells is IL-1b-dependent. (a) PT45-P1 and PT45-P1res cells were either left untreated or treated with 20 mg/ml etoposide for 48 h. Additionally, PT45-P1res cells were coincubated with 250 ng/ml IL1-RA. Cellular lysates were submitted to Western blotting using antibodies for the detection of full length and cleaved caspase-3, - 7, -8 and -9. An a-tubulin antibody was used as a control for equal protein load. (b) Cells were treated as above and analysed for caspase-3/7 activity expressed as n-fold induction of caspase-3/7 activity over basal. Means7s.d. from three independent experi- ments are shown. *Indicates Po0.05. (c) Detection of <t>cIAP1,</t> cIAP2 and XIAP in untreated cellular lysates of PT45-P1 and PT45-P1res cells by Western blotting. An a-tubulin antibody was used as a control for equal protein load.
Goat Anti Human Ciap1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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R&D Systems goat polyclonal anti ciap1
Figure 3 Decreased caspase activation in PT45-P1res cells is IL-1b-dependent. (a) PT45-P1 and PT45-P1res cells were either left untreated or treated with 20 mg/ml etoposide for 48 h. Additionally, PT45-P1res cells were coincubated with 250 ng/ml IL1-RA. Cellular lysates were submitted to Western blotting using antibodies for the detection of full length and cleaved caspase-3, - 7, -8 and -9. An a-tubulin antibody was used as a control for equal protein load. (b) Cells were treated as above and analysed for caspase-3/7 activity expressed as n-fold induction of caspase-3/7 activity over basal. Means7s.d. from three independent experi- ments are shown. *Indicates Po0.05. (c) Detection of <t>cIAP1,</t> cIAP2 and XIAP in untreated cellular lysates of PT45-P1 and PT45-P1res cells by Western blotting. An a-tubulin antibody was used as a control for equal protein load.
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Santa Cruz Biotechnology goat polyclonal anti ciap1 2 antibody
Figure 3 Decreased caspase activation in PT45-P1res cells is IL-1b-dependent. (a) PT45-P1 and PT45-P1res cells were either left untreated or treated with 20 mg/ml etoposide for 48 h. Additionally, PT45-P1res cells were coincubated with 250 ng/ml IL1-RA. Cellular lysates were submitted to Western blotting using antibodies for the detection of full length and cleaved caspase-3, - 7, -8 and -9. An a-tubulin antibody was used as a control for equal protein load. (b) Cells were treated as above and analysed for caspase-3/7 activity expressed as n-fold induction of caspase-3/7 activity over basal. Means7s.d. from three independent experi- ments are shown. *Indicates Po0.05. (c) Detection of <t>cIAP1,</t> cIAP2 and XIAP in untreated cellular lysates of PT45-P1 and PT45-P1res cells by Western blotting. An a-tubulin antibody was used as a control for equal protein load.
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Bio-Techne corporation human/mouse bid antibody
Figure 3 Decreased caspase activation in PT45-P1res cells is IL-1b-dependent. (a) PT45-P1 and PT45-P1res cells were either left untreated or treated with 20 mg/ml etoposide for 48 h. Additionally, PT45-P1res cells were coincubated with 250 ng/ml IL1-RA. Cellular lysates were submitted to Western blotting using antibodies for the detection of full length and cleaved caspase-3, - 7, -8 and -9. An a-tubulin antibody was used as a control for equal protein load. (b) Cells were treated as above and analysed for caspase-3/7 activity expressed as n-fold induction of caspase-3/7 activity over basal. Means7s.d. from three independent experi- ments are shown. *Indicates Po0.05. (c) Detection of <t>cIAP1,</t> cIAP2 and XIAP in untreated cellular lysates of PT45-P1 and PT45-P1res cells by Western blotting. An a-tubulin antibody was used as a control for equal protein load.
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Bio-Techne corporation normal goat igg control
Figure 3 Decreased caspase activation in PT45-P1res cells is IL-1b-dependent. (a) PT45-P1 and PT45-P1res cells were either left untreated or treated with 20 mg/ml etoposide for 48 h. Additionally, PT45-P1res cells were coincubated with 250 ng/ml IL1-RA. Cellular lysates were submitted to Western blotting using antibodies for the detection of full length and cleaved caspase-3, - 7, -8 and -9. An a-tubulin antibody was used as a control for equal protein load. (b) Cells were treated as above and analysed for caspase-3/7 activity expressed as n-fold induction of caspase-3/7 activity over basal. Means7s.d. from three independent experi- ments are shown. *Indicates Po0.05. (c) Detection of <t>cIAP1,</t> cIAP2 and XIAP in untreated cellular lysates of PT45-P1 and PT45-P1res cells by Western blotting. An a-tubulin antibody was used as a control for equal protein load.
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Meso Scale Diagnostics LLC streptavidin-coated meso scale discovery (msd) plates
Figure 3 Decreased caspase activation in PT45-P1res cells is IL-1b-dependent. (a) PT45-P1 and PT45-P1res cells were either left untreated or treated with 20 mg/ml etoposide for 48 h. Additionally, PT45-P1res cells were coincubated with 250 ng/ml IL1-RA. Cellular lysates were submitted to Western blotting using antibodies for the detection of full length and cleaved caspase-3, - 7, -8 and -9. An a-tubulin antibody was used as a control for equal protein load. (b) Cells were treated as above and analysed for caspase-3/7 activity expressed as n-fold induction of caspase-3/7 activity over basal. Means7s.d. from three independent experi- ments are shown. *Indicates Po0.05. (c) Detection of <t>cIAP1,</t> cIAP2 and XIAP in untreated cellular lysates of PT45-P1 and PT45-P1res cells by Western blotting. An a-tubulin antibody was used as a control for equal protein load.
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SouthernBiotech goat anti mouse
Figure 3 Decreased caspase activation in PT45-P1res cells is IL-1b-dependent. (a) PT45-P1 and PT45-P1res cells were either left untreated or treated with 20 mg/ml etoposide for 48 h. Additionally, PT45-P1res cells were coincubated with 250 ng/ml IL1-RA. Cellular lysates were submitted to Western blotting using antibodies for the detection of full length and cleaved caspase-3, - 7, -8 and -9. An a-tubulin antibody was used as a control for equal protein load. (b) Cells were treated as above and analysed for caspase-3/7 activity expressed as n-fold induction of caspase-3/7 activity over basal. Means7s.d. from three independent experi- ments are shown. *Indicates Po0.05. (c) Detection of <t>cIAP1,</t> cIAP2 and XIAP in untreated cellular lysates of PT45-P1 and PT45-P1res cells by Western blotting. An a-tubulin antibody was used as a control for equal protein load.
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Bio-Techne corporation rabbit igg horseradish peroxidase-conjugated antibody
Figure 3 Decreased caspase activation in PT45-P1res cells is IL-1b-dependent. (a) PT45-P1 and PT45-P1res cells were either left untreated or treated with 20 mg/ml etoposide for 48 h. Additionally, PT45-P1res cells were coincubated with 250 ng/ml IL1-RA. Cellular lysates were submitted to Western blotting using antibodies for the detection of full length and cleaved caspase-3, - 7, -8 and -9. An a-tubulin antibody was used as a control for equal protein load. (b) Cells were treated as above and analysed for caspase-3/7 activity expressed as n-fold induction of caspase-3/7 activity over basal. Means7s.d. from three independent experi- ments are shown. *Indicates Po0.05. (c) Detection of <t>cIAP1,</t> cIAP2 and XIAP in untreated cellular lysates of PT45-P1 and PT45-P1res cells by Western blotting. An a-tubulin antibody was used as a control for equal protein load.
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Image Search Results


Figure 3 Decreased caspase activation in PT45-P1res cells is IL-1b-dependent. (a) PT45-P1 and PT45-P1res cells were either left untreated or treated with 20 mg/ml etoposide for 48 h. Additionally, PT45-P1res cells were coincubated with 250 ng/ml IL1-RA. Cellular lysates were submitted to Western blotting using antibodies for the detection of full length and cleaved caspase-3, - 7, -8 and -9. An a-tubulin antibody was used as a control for equal protein load. (b) Cells were treated as above and analysed for caspase-3/7 activity expressed as n-fold induction of caspase-3/7 activity over basal. Means7s.d. from three independent experi- ments are shown. *Indicates Po0.05. (c) Detection of cIAP1, cIAP2 and XIAP in untreated cellular lysates of PT45-P1 and PT45-P1res cells by Western blotting. An a-tubulin antibody was used as a control for equal protein load.

Journal: Oncogene

Article Title: Acquired chemoresistance in pancreatic carcinoma cells: induced secretion of IL-1beta and NO lead to inactivation of caspases.

doi: 10.1038/sj.onc.1209423

Figure Lengend Snippet: Figure 3 Decreased caspase activation in PT45-P1res cells is IL-1b-dependent. (a) PT45-P1 and PT45-P1res cells were either left untreated or treated with 20 mg/ml etoposide for 48 h. Additionally, PT45-P1res cells were coincubated with 250 ng/ml IL1-RA. Cellular lysates were submitted to Western blotting using antibodies for the detection of full length and cleaved caspase-3, - 7, -8 and -9. An a-tubulin antibody was used as a control for equal protein load. (b) Cells were treated as above and analysed for caspase-3/7 activity expressed as n-fold induction of caspase-3/7 activity over basal. Means7s.d. from three independent experi- ments are shown. *Indicates Po0.05. (c) Detection of cIAP1, cIAP2 and XIAP in untreated cellular lysates of PT45-P1 and PT45-P1res cells by Western blotting. An a-tubulin antibody was used as a control for equal protein load.

Article Snippet: Detection of the IAP was performed using a goat anti-XIAP and a goat anti-cIAP1 antibody (both from R&D Systems) and a rabbit anti-cIAP2 antibody (Chemicon), each diluted at 1:1000 in 5% blotto-TBST.

Techniques: Activation Assay, Western Blot, Control, Activity Assay