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Image Search Results
Journal: Oncogene
Article Title: Acquired chemoresistance in pancreatic carcinoma cells: induced secretion of IL-1beta and NO lead to inactivation of caspases.
doi: 10.1038/sj.onc.1209423
Figure Lengend Snippet: Figure 3 Decreased caspase activation in PT45-P1res cells is IL-1b-dependent. (a) PT45-P1 and PT45-P1res cells were either left untreated or treated with 20 mg/ml etoposide for 48 h. Additionally, PT45-P1res cells were coincubated with 250 ng/ml IL1-RA. Cellular lysates were submitted to Western blotting using antibodies for the detection of full length and cleaved caspase-3, - 7, -8 and -9. An a-tubulin antibody was used as a control for equal protein load. (b) Cells were treated as above and analysed for caspase-3/7 activity expressed as n-fold induction of caspase-3/7 activity over basal. Means7s.d. from three independent experi- ments are shown. *Indicates Po0.05. (c) Detection of cIAP1, cIAP2 and XIAP in untreated cellular lysates of PT45-P1 and PT45-P1res cells by Western blotting. An a-tubulin antibody was used as a control for equal protein load.
Article Snippet: Detection of the IAP was performed using a goat anti-XIAP and a
Techniques: Activation Assay, Western Blot, Control, Activity Assay